冷嘉鹏

Email: lengjp1985@163.com

手机:+86 13509710431

性别：男

出生年月：1985.12

工作经历

2018年9月-至今北卡罗来纳大学教堂山分校，博士后研究员

2016年1月至2018年9月加利福尼亚大学河滨分校，博士后研究员

2013年10月-至今中北大学化学工程与技术学院讲师

教育经历

2008年9月-2013年6月 中国科学院上海有机化学研究所国家大型仪器分析中心

—上海质谱中心分析化学博士

2004年9月-2008年6月 沈阳药科大学 药学基地班学士

研究工作简介

本人的研究方向集中于基于色谱质谱的分析化学、药物代谢和药物分析学、核酸修饰与损伤组学分析、代谢组学、蛋白质组学、基因毒性物质暴露组学。具有多年质谱，色谱分析经验，熟悉多种色谱质谱仪及数据分析。发展和运用新型质谱技术，筛选和定量蛋白质/DNA修饰，了解脂质过氧化的健康影响，设计策略减少暴露相关的不良反应。研究生物活性多肽、蛋白质核酸加合物组学，以回答生物大分子如何与风险毒性物质相互作用，及相关风险评估。

通过稳定同位素标记和超灵敏质谱法对风险物质暴露所致核酸-蛋白质交联和蛋白质加合物进行结构鉴定。采用高度整合的系统分析方法，包括代谢组学，蛋白质组学和脂质组学，各种细胞动物和疾病模型的应用，研究与评估风险物质暴露损伤。此外还注重将所开发的新型有机分析手段与策略，应用于药物安全性评价方法开发、食品安全检测和活性生物分子分析等。

现任Journal of Integrative Oncology, Journal of Hematology and Oncology Forecast, Austin Journal of Nutrition and Food sciences, Journal of Oncology Research Forecast编委。至今已发表22篇科研论文，授权两项发明专利。国际知名学术期刊：Analytica Chimca Acta，Talanta，Journal of Chromatography B，Rapid communication of mass spectrometry，PeerJ—the Journal of Life and Environmental Sciences，Biomedical Chromatography，Journal of Separation Science，Biochemistry and Biophysics Report，International Journal of Mass Spectrometry, Nature出版集团刊物Commuications Biology特邀审稿人。

主持的科研项目及人才基金项目情况

1.国家自然科学基金青年基金，批准号：21904121、项目名称：羧基同位素化学标记辅助的DNA加合物质谱分析方法开发及应用，资助年限：2020/01-2022/12、在研、主持。

2.国家自然科学基金面上项目，批准号：21272262、项目名称：基于天然纳米材料膨润土的质谱新技术与新方法的研究、资助年限：2013/01-2016/12、已结题、主要参加人员。

3.国家自然科学基金面上项目，批准号：21275155、项目名称：质谱分析导向的低残留稳定同位素季胺化标记技术研究、资助年限：2013/01-2016/12、已结题、主要参加人员。

4.国家自然科学基金面上项目，批准号：21172250、项目名称：质谱法研究Lewis酸催化/促进的碳-碳键、碳-氮键形成反应机理、资助年限：2012/01-2015/12、已结题、参与。

5.国家自然科学基金面上项目，批准号：21072215、项目名称：质谱学方法研究有机化学反应中的瞬态自由基、资助年限：2010/01-2013/12、已结题、参与。

语言技能

具有良好的英语听、说、读、写能力，普通话二级甲等。

发表论文

1. Leng, J.; Liu, C.W.; Hartwell, H.J.; Yu, R.; Lai, Y.; Bodnar, W.M., et al. Evaluation of inhaled low-dose formaldehyde-induced DNA adducts and DNA-protein cross-links by liquid chromatography-tandem mass spectrometry. Archive of Toxicology. 93:763-773; 2019

2. Leng, J.; Wang, Y. Liquid Chromatography-Tandem Mass Spectrometry for the Quantification of Tobacco-Specific Nitrosamine-Induced DNA Adducts in Mammalian Cells. Analytical Chemistry. 89:9124-9130; 2017

3. Guo, S.;Leng, J.; Tan, Y.; Price, N.E.; Wang, Y. Quantification of DNA Lesions induced by 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanol in Mammalian Cells. Chem Res Toxicol. 32: 708-717; 2019

4. Leng, J.; Guan, Q.; Sun, T.; Wang, H.; Cui, J.; Liu, Q., et al. Direct infusion electrospray ionization-ion mobility-mass spectrometry for comparative profiling of fatty acids based on stable isotope labeling. Anal Chim Acta. 887:148-154; 2015

5. Leng, J.; Wang, H.; Zhang, L.; Zhang, J.; Guo, Y. A highly sensitive isotope-coded derivatization method and its application for the mass spectrometric analysis of analytes containing the carboxyl group. Anal Chim Acta. 758:114-121; 2013

6. Leng, J.; Guan, Q.; Sun, T.; Wu, Y.; Cao, Y.; Guo, Y. Application of isotope-based carboxy group derivatization in LC-MS/MS analysis of tissue free-fatty acids for thyroid carcinoma. J Pharm Biomed Anal. 84:256-262; 2013

7. Leng, J.; Zhu, D.; Wu, D.; Zhu, T.; Zhao, N.; Guo, Y. Analysis of the differentially expressed low molecular weight peptides in human serum via an N-terminal isotope labeling technique combining nano-liquid chromatography/matrix-assisted laser desorption/ionization mass spectrometry. Rapid Commun Mass Spectrom. 26:2555-2562; 2012

8. Leng, J.; Wang, H.; Zhang, L.; Zhang, J.; Cai, T.; Yao, J., et al. Integration of high accuracy N-terminus identification in peptide sequencing and comparative protein analysis via isothiocyanate-based isotope labeling reagent with ESI ion-trap TOF MS. J Am Soc Mass Spectrom. 22:1204-1213; 2011

9. Leng, J.; Wang, H.; Zhang, L.; Zhang, J.; Guo, Y. Application of Tandem Mass Spectra for the Peaks of Isotope Pattern in the Elemental Composition Assignment of Product Ions. Chin J Chem. 28:1751-1756; 2010

10. Leng, J.; Liu, C.W.; Hartwell, H.J.; Yu, R.; Lai, Y., et al. Evaluation of Inhaled Low Dose Formaldehyde Induced DNA Damage by Liquid Chromatography-Tandem Mass Spectrometry. Toxicol. Lett. 295:S155-S156; 2018 (Abstract)

11. Wang, P.;Leng, J.; Wang, Y. DNA replication studies of N-nitroso compound-induced O (6)-alkyl-2'-deoxyguanosine lesions in Escherichia coli. J Biol Chem. 2019:007358; 2019

12. Du, H.;Leng, J.; Wang, P.; Li, L.; Wang, Y. Impact of tobacco-specific nitrosamine-derived DNA adducts on the efficiency and fidelity of DNA replication in human cells. J Biol Chem. 293:11100-11108; 2018

13. Zhou, T.;Leng, J.; Peng, Y.; Zhang, L.; Guo, Y. Mass spectrometric analysis of free fatty acids in infant milk powders by frozen pretreatment coupled with isotope-labeling derivatization. J Sep Sci. 39:873-879; 2016

14. Liu, C.W.; Tian, X.; Hartwell, H.J.;Leng, J.; Chi, L.; Lu, K., et al. Accurate Measurement of Formaldehyde-Induced DNA-Protein Cross-Links by High-Resolution Orbitrap Mass Spectrometry. Chem Res Toxicol. 31:350-357; 2018

15. Fan, R.J.; Guan, Q.; Zhang, F.;Leng, J.; Sun, T.Q.; Guo, Y.L. Benzylic rearrangement stable isotope labeling for quantitation of guanidino and ureido compounds in thyroid tissues by liquid chromatography-electrospray ionization mass spectrometry. Anal Chim Acta. 908:132-140; 2016

16. Liu, Q.; Guo, J.; Cai, Y.;Leng, J.; Liu, H.; Chen, L. Preparation and Characterization of Aminated Silica Fluorescent Microspheres. Chemical Industry and Engineering Progress. 7:2130-2134; 2016.

17. Liu, Q.; Guo, J.;Leng, J.; Duan, P.; Liu, H.; Chen, L. Preparation and Characterization of Aminated Silica Fluorescent Microspheres. New Chemical Materials. 1: 98-100; 2016. (in

18. Cao, Y.; Guan, Q.; Sun, T.; Wang, H.;Leng, J.; Guo, Y. N-alkylpyridinium quaternization combined with liquid chromatography-electrospray ionization-tandem mass spectrometry: A highly sensitive method to quantify fatty alcohols in thyroid tissues. Anal Chim Acta. 849:19-26; 2014

19. Wang, H.; Zhang, L.; Zhang, J.;Leng, J.; Cai, T.; Guo, Y. N-alkylpyridinium quaternization for assisting electrospray ionization of sterols in oil by quadrupole-time of flight mass spectrometry. J Mass Spectrom. 48:1101-1108; 2013

20. Wang, H.; Zhang, L.; Zhang, J.;Leng, J.; Cai, T.; Guo, Y. Improvement and extension of the application scope for matrix-assisted laser desorption/ionization mass spectrometric analysis-oriented N-alkylpyridinium isotope quaternization. Anal Chim Acta. 707:100-106; 2011

21. Xue, J.; Lai, Y.; Chi, L.; Tu, P.;Leng, J.; Liu, C.W., et al. Serum Metabolomics Reveals That Gut Microbiome Perturbation Mediates Metabolic Disruption Induced by Arsenic Exposure in Mice. J Proteome Res. 18:1006-1018; 2019

22. Tu, P.; Xue, J.; Bian, X.; Chi, L.; Gao, B.;Leng, J., et al. Dietary administration of black raspberries modulates arsenic biotransformation and reduces urinary 8-oxo-2'-deoxyguanosine in mice. Toxicol Appl Pharmacol. 377:114633; 2019

授权专利

1.郭寅龙、冷嘉鹏等。一种同位素标记试剂及其制备方法和应用。中国专利，专利号：CN102174025B。

2.郭寅龙、冷嘉鹏等。哌嗪基嘧啶类同位素标记试剂的用途。中国专利，专利号：CN102944604B。